Responses of smooth muscle cells to platelet-derived growth factor are inhibited by herbimycin-A tyrosine kinase inhibitor .

Fujio Y ; Yamada F ; Takahashi K ; Shibata N

Center for Adult Diseases , Osaka , Japan .

Biochem Biophys Res Commun 195 : 79-83 ( 1993)

Abstract
Platelet-derived growth factor ( PDGF ) plays important roles in proliferation of smooth muscle cells ( SMCs ) . PDGF is also known to induce the cytoskeletal reorganization as a chemoattractant . In this communication , we demonstrate that the tyrosine kinase inhibitor herbimycin-A ( HM-A ) inhibited the effects of PDGF on SMCs in proliferation , phenotypic modulation , and reorganization of F-actin . Another tyrosine kinase inhibitor genistein also inhibited reorganization of F-actin . Since both tyrosine kinase inhibitors reduced PDGF-dependent tyrosine phosphorylation , tyrosine kinase activities may be involved not only in proliferation but also in reorganization of F-actin .