Participation of diacylglycerol kinase in mitogenic signal transduction induced by platelet-derived growth factor in vascular smooth muscle cells .

Inui H ; Kondo T ; Konishi F ; Kitami Y ; Inagami T

Department of Biochemistry , Vanderbilt University School of Medicine , Nashville , TN 37232 .

Biochem Biophys Res Commun 205 : 1338-44 ( 1994)

Abstract
In rat vascular smooth muscle cells , platelet-derived growth factor ( PDGF ) , stimulated phosphatidic acid synthesis by activating both of the two alternative pathways , diacyglycerol kinase ( DGK ) and phospholipase D ( PLD ) . Genistein , a tyrosine kinase inhibitor , inhibited PLD activation but not DGK activation , the latter was inhibited selectively by R 59022 . PDGF-induced DNA synthesis was partially inhibited by genistein or R 59022 , but these inhibitors had no effect on phorbol ester-induced DNA synthesis . Further , the specific effects of these inhibitors on PDGF-induced DNA synthesis were additive .